You want to clone actin gene into the EcoRI sites of the vec

You want to clone actin gene into the EcoRI sites of the vector above. You found that the both ends of actin gene in the human and mouse genome is flanked by EcoRI sites. Describe your plan of cloning actin gene into the vector. If necessary, draw the steps of cloning.

Solution

since both the vector and gene have EcoRI sites, the cloning would be relatively easy.

add EcoRI to the vector. the vector woud be opened at EcoRI sites.

add EcoRI to the gene. the desired actin gene would have EcoRI specific overhangs at both the ends.

mix the above two solutions along with ligases. since both the vectors and gene have same EcoRI overhangs, the gene would complementarily bind to the overhangs of vector.