If you can explain how you do these 3 questions as well I wo

If you can explain how you do these 3 questions as well I would greatly appreaciate it

1. A student is asked to characterise six yeast strains labelled 1, 2, 3, 4, 5 and 6. The student begins by streaking cells of each strain onto nine different tester media shown in Table I. Table l: Growth responses of the six strains on different tester media strain YEPD MM MM A MM L MM H MM +A L L H H H Legend growth and no growth, MM minimal medium, YEPD complete medium L leucine, A- arginine, and H histidine (each is an end product of a separate biochemical pathway, all are required for growth i) Using only the data from Table i, determine the genotype of each strain, using the same format as the wildtype strain. For this question only, assume there is only 1 gene/ pathway ie. Only 1 enzyme involved in making histindine, one enzyme to make arginine, etc. (3 pts) Strain Genotype Strain Genotype Wildtype arg leu his 6

Solution

1. Genotypes of the strains are:

Wild type: arg⁺; leu⁺; his⁺

1. arg^-; leu^-; his^-

2. arg⁺; leu⁺; his^-

3. arg^-; leu⁺; his⁺

4. arg^-; leu^-; his^-

5. arg⁺; leu^-; his⁺

6. arg^-; leu⁺; his⁺

Q2. Complementation groups

1. Strain 1 and 4

2. Strain 2

3. Strain 3

4. Strain 5

5. Strain 6

Q 2b. Total 6 genes are mutated.

Q3.

Genotypes of:

Strain 1- arg-1; ARG2; LEU-2; leu 2; HIS1; his2

Strain 3-.arg-1; ARG-2; LEU-1; LEU-2; HIS-1; HIS-2

Strain 4- Arg-1; arg-2; leu-1; LEU-2; his-1; HIS-2