you swabbed a PE agar plate and an LB agar plate with an E C
you swabbed a PE agar plate and an LB agar plate with an E Coli culture. after 24 hours incubation period at 37 C both plates show the sae amount of growth. did you expect this result? if not give detailed explanation
Solution
LB and PE agar is mainly employed for E.coli cell growth and propagation. LB may help E.coli growth up to a cell density of OD600 = 2 to 3. High density growth media may increase DNA and protein yields by raising mobile density. LB includes the enzymatic digestion item of casein frequently known as peptone (some sellers contact it Tryptone), yeast extract, and sodium chloride. Peptone is casein digested by pepsin and Tryptone is casein digested by trypsin. We don\'t discover significant difference between these two casein digestions in E.coli cultures. Peptone is rich in proteins and peptides. Their amino acid and peptide compositions reveal those of casein. In addition to proteins and peptides, yeast extract also contains nucleic acids, lipids and different nutritional elements which are essential to E.coli mobile growth.
