What can you conclude about the protein profile of fat free

What can you conclude about the protein profile of fat free vs whole milk? What can you conclude about the protein profile of whole milk and soy milk? What sizes are the predominant proteins in cow\'s milk? Soy milk? Why could your graph be plotted concentration (mg/ml) of protein vs absorbance OR mg of protein vs absorbance? How would each plot affect your sample preparation and reading?

Solution

1, The separation of proteins in fat-free milk are resolved better than in the whole milk. The fat in the milk seems to interfere with the protein separation on the polyacrylamide gel. The wells are highlighted in the case of the whole milk or in milk with low fat.

2. Whole milk versus Soy Milk

The cow milk has fewer proteins as compared with the soy milk. Soy milk have broad range of protein i.e., from 10 to 77 kDa while cow milk has smaller molecular weight proteins only (ranging from 10- 30 kDa)

3. Sizes of proteins predominant in the cow milk:

Molecular weight- 17, 28 and 30 kDa

Sizes of proteins predominant in the soy milk:

Molecular weight- 18, 35 and 75kDa

4. The graph could be plotted concentration of protein vs absorbance because the absorbance is directly proportional to the concentration of the protein. The independent variable concentration can be taken on X-axis and the dependent variable absorbance is taken on the Y-axis.

The equal amount of protein (mg) is loaded per well of the polyacrylamide gel. Therefore, the plot telling the concentration of the protein would affect the sample preparation and reading.