3 The nucleotide sequences of the DNA molecules below are th

3. The nucleotide sequences of the DNA molecules below are the same except for a single point mutation highlighted in bold.

DNA sequence 5’-ATG ATC GGA TCG ATC CAT-3’

3’-TAC TAG CCT AGC TAG GTA-5’

a) Transcribe the sequence (Hint: RNA polymerization is 5’ 3’, complementary to the 3’ 5’ template)

b) Translate the sequence using the genetic code table.

Solution

ANSWER :

Given DNA sequence

5’-ATG ATC GGA TCG ATC CAT-3’

3’-TAC TAG CCT AGC TAG GTA-5’

a)Transcribed mRNA sequence 5-AUG AUC GGA UCG AUG CAU 3\'

b) Amino acid sequence N-terminal Met - Ile - Gly - Ser - Met - His C-terminal   

RNA polymerase elongates an RNA strand by adding ribonucleotide units to the 3-hydroxyl end, building RNA
in the 5\' - 3\' direction.