See image Please explain to me why the answer is B since I t

See image. Please explain to me why the answer is B since I thought okazaki fragments would not be produced in vitro, and thus no need to connect them.

1. DNA polymerase l s the first DNA polym discovered in E.coli. This enzyme has 5\' to 3\' erase polymerase activity, 5 to 3\' exonuclease activity and 3 to 5\' exonuclease activity in vitro. During purification of DNA polymerase I, its 5\' to 3\' exonuclease activity is lost, If we use the purified enzyme for an in vitro DNA re action reaction, where all other components are provided, which of the following is a most likely outcome? fr down. DNA replication will proceed normally. Okazaki fragments will be generated, but will not be connected. C. DNA replication will not initiate. X The new DNA strands will have a lot of mistakes in them

Solution

Yes, you are right. okazaki fragments formation does not occurs in vitro. but In this case okazaki fragments will be generated bacause here it is clearily mentioned that during purification of DNA polymerase I its 5\' to 3\' activity is lost. Thats why when replication process will take place the newly formed 3\' to 5\' strand will be continous strand and 5\' to 3\' strand will be formed discontinously as okazaki fragments.