Choose the accurate answer To view live cells in a dash we n

Choose the accurate answer To view live cells in a dash we need a/in inverted microscope up-right microscope electron microscope good microscope Blood serum must be added to mammals an cell media because, it has nutrient components it has growth factors & signaling molecules that include cell growth. Mammalian cells need blood Blood serum is required for oxygen &CO;_2 transport mammalian cells grows until they cover the entire surface of the first of the duty/flask where they touch on another point they stop growing this phenomenon is correctly known as Overgrowth inheritance touching inhibition contact inhibition growth factor-induced inhibition. Choose the best accurate a when cells crowded on the dish with no or very little spaces between them, we are them as being crowded saturated overgrown confluent when cells stop growing as described in questions 19 & 20 above, to prevent excessive cell death they must be _____ discarded in biohazard container Fed with more rich media to enhance robust growth Provided with growth factors Passage choose the most accurate answer: To split mammalian first we aspirate the media, cells, then we wash with 1X PBS and apply try sin wash with 1X PBS and apply media Wash with buffer and apply try sin wash with 1X PBS and apply pepsin

Solution

1. C. Electron microscope because of its high magnification and resolution.

2. B. It has growth factors and signalling molecules that induce cell growth; self explanatory.

3. C. Contact inhibition, a regulatory process that is absent in cancerous cells which continue to replicate whereas in the normal process the division process stops when the contact is established.

4. B. Saturated; self explanatory

5. D. Passaged by shaking the flask which would result in mixing of oxygen and prevent biofilm formation.

6. A. Was with 1X PBS and apply trypsin, and it is the procedure.