Can the size of a supercoiled plasmid DNA be determined by u

Can the size of a supercoiled plasmid DNA be determined by using standard DNA size fragment electrophoresed in parallel?

Solution

SUPERCOILED PLASMID DNA:Plasmid is coiled histone like proteins.in vitro,the proteins are stripped away during extraction from the bacterial cell making plasmid molecule to super coil itself.

STANDARD DNA:The sizes of DNA in nucleiacid electrophoresis is accompalished by comparision to size standards called ladders or makers called standard dna.

NO,the size of a supercoiled plasmid DNA be determined by using standard DNA size fragment electrophoresed in parrel.

Explanation:supercoiled plasmid DNA have circular structures ,that means, one DNA is super coild and other is relaxed(forming differentstates of relaxation).the migration factor form double bands of plasmids in electrophoresis making them compare to hard in parallel in standard marker. plasmids can be cut with endonuclease restrications by once or twice before performing electrophoresis you can draw restrication map and measure the plasmid size on the standard DNA marker size in parallel.

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