Unsure on how to calculate NaCl concentration Shown below is
Unsure on how to calculate NaCl concentration????
Shown below is a dilution protocol of a 5M stock solution of NaCI. The lines above indicate how much volume you are transferring to the volume of water Indicated in the tube. For each tube state the dilution factor and concentration of NaCl.Solution
Answer:
Dilution is defined as reduction of the concentration of a solution by adding diluent such as water. And the Dilution factor is the ratio of the initial volume (of solute/solution) to the final volume of any solution.
In the given case, the 5M NaCl is to be diluted using water as diluent.
Thus, dilution factor for tube A = 5/(5+5) = 1:2
The dilution factor for tube B = 2.5/(2.5+7.5) = 2.5/10 = 1:4
The dilution factor for tube C = 3/ (3+3) = 3/6 = 1:2
The final concentration of a solution can be calculate using the following formula:
N1V1 = N2V2
where as, N1 is the initial concentration of the solution
N2 is the final concentration of the solution
V1 is the initial volume of the solution
V2 is the final volume of the solution
For tube A, N1 = 5M; V1= 5 ml; V2 = 10 ml; N2 = ?
N2 = N1V1/V2
= (5M x 5 ml) / 10 ml
= 25/10 = 2.5 M
For tube B, N1 = 2.5M; V1= 2.5 ml; V2 = 10 ml; N2 = ?
N2 = N1V1/V2
= (2.5M x 2.5 ml) / 10 ml
= 6.25/10 = 0.625 M
For tube C, N1 = 0.625M; V1= 3 ml; V2 = 6 ml; N2 = ?
N2 = N1V1/V2
= (0.625M x 3 ml) / 6 ml =1.875 / 6
= 0.3125 M
