Homework SourseA question in biochemistry Why must the mass of dextran blue
A question in biochemistry,
Why must the mass of dextran blue be much larger than 80 000 Da in order to calculate for Vi ( inner volume)
A question in biochemistry,
Why must the mass of dextran blue be much larger than 80 000 Da in order to calculate for Vi ( inner volume)
Why must the mass of dextran blue be much larger than 80 000 Da in order to calculate for Vi ( inner volume)
Solution
Size exclusion chromatography separates molecules on the basis of the size and shape. Size of the blue dextran is 2*10(6)g/mol. Vi is the inside volume of the porous beads that is accessible to the small molecules because the separation of proteins depends on the porous size of the beads. The selection of materials is based on the size of proteins which has to be separated.
