What is a Sandwich assay give an exampleSolutionThe analyte

What is a Sandwich assay, give an example?

Solution

The analyte in the unknown sample is bound to the antibody site, then labelled antibody is bound to the analyte. The amount of labelled antibody on the site is then measured. It will be directly proportional to the concentration of the analyte because labelled antibody will not bind if the analyte is not present in the unknown sample. This type is also known as sandwich assay as the analyte is \"sandwiched\" between two antibodies.

an example of sandwitch assay is ELISA ((enzyme-linked immunosorbent assay)