What are the limitations to the common techniques in detecti

What are the limitations to the common techniques in detecting the presence of a class of macro-molecules? Do biologists who study plant cells commonly use the iodine test for starch? why or why not? Test/techniques : -Benedict\'s test -Iodine test - Biuret test -dische diphenylamine

Solution

The macromolecules are every large molecule such as proteins, carbohydrates, lipids , etc. Various tests are performed in the labs to detect the presence of these macromolecules in the food we consume. The main limitations of these tests are listed below:

Benedict\'s Test: The Benedict test is used to detect the simple carbohydrates. Its limitations are as follows:

Iodine Test: Iodine test is used to detect the presence of starch. The limitation for iodine test are as follows:

Biuret Test: This test is used to detect the presence of peptide bond, that is for the proteins. The main limitations are as follows:

Biologists stain plant cells with iodine: Iodine reacts with starch in the cell causing the change in color and allowing to see it through the microscope. The color of iodine changes from yellowish brown to blackish blue. Amylose in the starch is responsible for the formation of a deep blue color in the presence of iodine.