1 What cuvet material is most appropriate for quantifying DN

1. What cuvet material is most appropriate for quantifying DNA, which absorbs at 260nm?

plastic

quartz

PVDF

any of the above materials would work

2. DEAE-cellulose resin

is positively charged at pH 8.0

is neutral at pH 8.0

is negatively charged at pH 8.0

can change its charge depending on the salt concentration

3.Which of the following is true of the membrane material used for our western blot?

It is negatively charged.

It is hydrophilic.

It is hydrophobic.

D. It reacts specifically with CDP-Star.

4. Why is bromophenol blue used in SDS-PAGE sample (loading) buffer?

to visualize the extent of electrophoresis

to reduce disulfide bonds of proteins

to generate free radicals for gel polymerization

to help the sample sink into the wells

5. Which of the following is true of our detection of ADH by western blot?

The primary antibody bound to the ADH portion of the protein.

The primary antibody bound to the histidine tag on the recombinant ADH protein.

The primary antibody bound to the strep tag on the MW standards.

None of the above

Ans 1. Quartz

Ans 2. Is negatively charged at 8 ph

Ans 3. Hydrophobic

Ans 4. To redude disulfide bond in protein

Ans 5. The primary antibody bound to ADH portion of the protein