Answer 4567891011 2 The following equation is the respiratio

Answer 4,5,6,7,8,9,10,11

2. The following equation is the respiration of glucose: a. What are the substrates? b. What are the products? c. Be able to identify the substrate(s), enzyme (s), and product(s) when represented an equation such as: CsH12Os 60, Acetyl CoA- 6CO2 6H20 3. What is formed when an enzyme binds with its substrate? tempora ry enzyme Sub 4. Where does the substrate bind to its respective enzyme? acti ve sire 5. How much of an enzyme is used up in a reaction? How much is recycled to repeat the reaction? 6. Why is it important to mix/invert the test tube of your enzyme experimental components? 7. What happens to a denatured enzyme? How can an enzyme become denatured? 8. What is activation energy (Ea)? How can it be reduced? 9. In experiment 4.1 you observed the difference in enzyme reaction rates based on their concentration a. What was the indicator that was used in the coupled reaction? b. What can you conclude about the relationship between enzyme concentration and reaction rate? c. What do you think would happen to all of the test tubes prepared in experiment 4.1 if you observed their absorbance after 24 hours instead of just a few minutes? Why? 10. How does the peroxidase enzyme function in a pH 7 buffer at 20 C? a. How does this reaction compare to peroxidase in pH 4 solution at 20 C? b. How does this reaction compare to peroxidase in pH 10 solution at 20 c? c. How does this reaction compare to peroxidase in pH 7 solution at 4 c? d. How does this reaction compare to peroxidase in pH 7 solution at 80 C? 11. The spectrophotometer was used to measure the absorbance of each enzyme reaction (Representing how much guaiacol had been oxidized). This absorbance reading was used as a proxy to represent what? (ie, what did A4To equate to in reference to the enzyme reaction?)

Solution

(4)Substrate binds to active site of the enzyme

(5)Enzymes are not consumed or produced in a reaction. Enzyme is the catalyst, it is not used up in the reaction. Enzymes become saturated when the substrate concentration is high.

(6)Inverting the test tube helps in mixing the components of the tube.

(7) Enzymes are proteins, when subjected to extreme environments such as high temperatures and high acidity they denature.

(8)Activation energy is the minimum amount of energy that is required for a reaction to happen. Enzymes lower the activation energy by bringing together the reacting molecules and facilitating the formation products.