For the following problems you need to know the ratio of the

For the following problems, you need to know the ratio of the diluent to the sample aliquot. For instance, if you are making a 1:20 dilution, the ratio of diluent to aliquot will be 1 unit less than the dilution factor, i.e., 20 parts total, 19 parts diluent, 1 part sample aliquot.

1) How much sample (i.e., what volume aliquot) is required to prepare 10 mL of a 1:10 (1 to 10) dilution? How much diluent would you need?

Solution

Dilution method is required to dilute the sample serially to make different dilution. The sample of 10mL is maintained as stock. From the stock, 1 mL is taken and added to 9mL diluent to make it as 10^-1 (1/10) dilution. Then 1 mL from 10^-1 dilution added to 9mL diluent results in 10^-2 dilution or 1/100 dilution. Likewise, the dilutions can be prepared as required.

To prepare 10mL 1: 10 dilution 1 mL sample and 9 mL diluent are required. it becomes 10^-1 dilution.