Your gel was a 06 gel in IX TAE buffer How much agarose how

Your gel was a 0.6% gel in IX TAE buffer. How much agarose, how much 60X TAE buffer and how much water would you combine to make 40mls of this gel? (Show ALL calculations) Fast Red is a dye that is similar in size and function to FastGreen, but is colored RED. Assuming that they were at the same concentration, would you expect FastRed and FastGreen to have the same absorbance at 550nm? Why or why not?

Solution

(8)

Required: 0.6%, 1X TAE buffer

Stock = 60x TAE

Required = 1x

required Volume = 40 mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (1x X 40 mL)/ 60x

= 0.6 mL

0.6 mL of stock + 39.4 mL of H2O gives 1x TAE

Agarose: 0.6%

Means 6g in 100 mL

For 40 mL -------> (40 x 6)/100 =2.4g

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2.4 g of agarose dissolved in 1x TAE