PCR RE lab questions Design a pair of primers to your gene

PCR & RE lab questions

Design a pair of primers to your gene of interest.  

Your PCR reaction shows no bands at all, give three reasons as to why that could happen.

The ladder indicates that the size difference between the blue and white colony plasmids is much smaller than it should be based on the size of your PCR product, what is a likely explanation?

Solution

Three reasons for no PCR amplification:

A much smaller size difference between the blue and white colony plasmids indicates that the complete insert was not introduced successfully in the vector (faulty cloning strategy, breaking of DNA, etc.).