The following fragments were obtained from the treatment of

The following fragments were obtained from the treatment of a peptide with standard protease enzymes. Given the following information write the correct peptide sequence.

(a) Thermolysin (cuts on the N side of Ile, Phe, Tye, Rn1Pro)

Ala,

Gly, Ile

Cys, Met, Phe, Pro

Arg, Ser, Tyr, Lys

(b) Aminorthylation followed by trypsin (cuts on the C side of Lys, Arg, AECys, Rn+1 Pro)

Lys,Ser

Phe, AECys, Pro, Ala

Ile, Tyr, Gly, Arg, Met

Solution

If thermolysin used and cuts an enzyme (Rn+1) after Proline , the sequence would be

Ala-Ile-Gly-Phe-Cys-Met-Pro-Arg-Ser-Lys-Tyr

For Aminorthylation followed by trypsin

Trypsin digestion can follow for Arginine and Lysine if they are not link to proline.

Ser-Lys- Phe-AEcys-Pro-Ala-Ile-Tyr-Gly-Arg-Met