This figure shows the rate of activity of the enzyme Hornase

This figure shows the rate of activity of the enzyme Hornase. Hornase catalyzes the reaction that converts Lukanate to Hornic Acid. The Km of this enzyme at this concentration is 6. The pH at which it works best is 6.8. What enzyme activity rate would you expect in a tube with 1mg of enzyme and 3 units of substrate?

Solution

Answer:

1 mg = 1000 micrograms/litre

Enzyme activity : Volume x Substrate conversion rate

                      = 1000 x 3

                      = 3000 units