The final result in RFLP analysis looks like a simplified su

The final result in RFLP analysis looks like a simplified supermarket bar code. The latter is absolutely unique. Is the RFLP pattern unique?

The final result in the PM + DQA1 test is seen as blue dots on a white strip. Why are the positive and negative controls so important in this test?

Solution

Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences which can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specific restriction endonucleases. It is highly locus specific used for the analysis of unique patterns in DNA fragments in order to genetically differentiate between organisms An RFLP probe is a labeled DNA sequence that hybridizes with one or more fragments of the digested DNA sample after they were separated by gel electrophoresis, revealing a unique blotting pattern characteristic specific to a genotype at a specific locus.

In PM+DQA1 positive control is important to see that correct amplicon is resulted from amplification. Negative control on the other hand will specify any kind of contamination of the sample