BIO: cell and molecular
ANSWER ALL
1 Match the conditions (of plasmid and plate) with the expected growth
+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid
-pGLO on LB/Ara
-pGLO on LB/Ara
-pGLO on LB/Amp
Growth of only white colonies, growth of both white colonies and green fluorescent colonies, no growth at all, growth of only green fluorescent colonies
2 correctly match the steps of the experiment. Pay attention as the steps may be jumbled.
•bacteria are plated on LB and grown overnight
•bacteria are placed in tubes with or without pGLO
•bacteria are plated on various plates and grown overnight
•bacteria are heated to 42 degrees C
•Single colonies of bacteria are picked for transformation
BIO: cell and molecular
ANSWER ALL
1 Match the conditions (of plasmid and plate) with the expected growth
+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid
-pGLO on LB/Ara
-pGLO on LB/Ara
-pGLO on LB/Amp
Growth of only white colonies, growth of both white colonies and green fluorescent colonies, no growth at all, growth of only green fluorescent colonies
2 correctly match the steps of the experiment. Pay attention as the steps may be jumbled.
•bacteria are plated on LB and grown overnight
•bacteria are placed in tubes with or without pGLO
•bacteria are plated on various plates and grown overnight
•bacteria are heated to 42 degrees C
•Single colonies of bacteria are picked for transformation
ANSWER ALL
1 Match the conditions (of plasmid and plate) with the expected growth
+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid
-pGLO on LB/Ara
-pGLO on LB/Ara
-pGLO on LB/Amp
Growth of only white colonies, growth of both white colonies and green fluorescent colonies, no growth at all, growth of only green fluorescent colonies
2 correctly match the steps of the experiment. Pay attention as the steps may be jumbled.
•bacteria are plated on LB and grown overnight
•bacteria are placed in tubes with or without pGLO
•bacteria are plated on various plates and grown overnight
•bacteria are heated to 42 degrees C
•Single colonies of bacteria are picked for transformation
1 Match the conditions (of plasmid and plate) with the expected growth
+pGLO indicates transformation by the pGLO plasmid
-pGLO indicates no plasmid
-pGLO on LB/Ara
-pGLO on LB/Ara
-pGLO on LB/Amp
Growth of only white colonies, growth of both white colonies and green fluorescent colonies, no growth at all, growth of only green fluorescent colonies
2 correctly match the steps of the experiment. Pay attention as the steps may be jumbled.
•bacteria are plated on LB and grown overnight
•bacteria are placed in tubes with or without pGLO
•bacteria are plated on various plates and grown overnight
•bacteria are heated to 42 degrees C
•Single colonies of bacteria are picked for transformation
(1)
-pGLO on LB/Ara
no growth at all
-pGLO on LB/Ara
no growth at all
-pGLO on LB/Amp
no growth at all
The –pGLO sign indicates that they did not get pGLO plasmid, so they cannot survive on the antibiotics like ampicillin.
The colonies which have got +pGLO will grow on antibiotic and also fluoresce due to the presence of GFP gene. Only the presence of sugar arabinose, The GFP gene will be turned on and the expression of GFP protein allows the cells to fluoresce in the presence of UVlight
(2)
•bacteria are plated on LB and grown overnight
•Single colonies of bacteria are picked for transformation
•bacteria are placed in tubes with or without pGLO
•bacteria are heated to 42 degrees C
•bacteria are plated on various plates and grown overnight
| -pGLO on LB/Ara | no growth at all |
| -pGLO on LB/Ara | no growth at all |
| -pGLO on LB/Amp | no growth at all |
Homework Sourse
