On the basis of the above data lit the media in order from b

On the basis of the above data, lit the media in order (from best to worst) according to their ability to support the growth of bacteria. List the three bacterial species in order of their increasing fastidiousness. Why did the most fastidious organism grow poorly in the chemically defined medium?

Solution

(1)

E.coli

Yeast extract broth> Nutrient Broth> Glucose Broth> Inorganic synthetic broth

A.faecalis

Yeast Extract > Broth> Nutrient Broth>Inorganic Synthetic broth> Glucose broth

S. mixa  

Yeast Extract Broth>   Inorganic Synthetic broth> Glucose broth>Nutrient broth

(2)The Inorganic Synthetic Broth is chemically defined medium. So based on the growth in this media, we can know the fastidiousness of any bacteria.

Increasing fastidiousness

S.mixa> E.coli>A.faecalis

(3)As we all know fastidious bacteria grow when it is provided with specific complex nutritional media

As you know, the fastidious organism will need specific nutrients to grow well, but chemically define medium cannot supply them, therefore the fastidious organisms will not grow well in chemically defined medium