You transform your bacteria with the GFP plasmid only to fin

You transform your bacteria with the GFP plasmid only to find that nothing grows on your agar plate. Give at least three explanations as to why this might have happened (pipetting error can not be one of them)?

Solution

Because gfp plasmid provide s resistance to the growth of other kind of microbes.

And it has some antibiotic kind of property with fluorescent color visible.

We can provide certain markers too as it is a plasmid by nature it will work as a vector which is a kind of beneficial to us..