Number the following steps of todays lactase enzyme assay in

Number the following steps of today\'s lactase enzyme assay in the correct order. Calculate amount of product formed from absorbance. Set up reaction tubes with buffer and substrate. Warm reaction tubes at 37 degree C. Measure absorbance of each reaction tube. Add 0.1 mL enzyme to each reaction tube, staggered at 30 second intervals. Add 1.0 mL K_2CO_3 to stop reaction. Incubate reaction tubes at 37 degree C for 10 minutes.

Solution

Lactase enzyme assay in correct order =

1] Set up reaction tubes with buffer & substrate

2] Add 0.1 ml enzyme to each reaction tube ,staggered at 30 second intervals.

3] Warm reaction tubes at 37^oC

4] Add 1.0 mL K₂CO₃ to stop reaction

5] Incubate reaction tubes at 37⁰C for 10 minutes

6] Measure the absorbance of each tubes

7] Calculate the amount of product formed from absorbance