You were assigned the CD4 T cell marker during the MACsFlow

You were assigned the CD4 T cell marker during the MACs/Flow Laboratory but by mistake you added 100lt of MACs anti CD4 antibody instead of 10lt prior to labeling these cells with FITCs anti CD4 antibody. Predict the % of the CD4 +ve cells, identified by Flow-Cytometry in the Negative and Positive selection fraction. Explain your reasoning

Solution

Many in vivo investigation and most of them incorporate CD4 T cell adoptive transport between mice. But not multiple results show notable dissimilarity and if the T cells I transport might be non-functioning.

My bother is that anti-CD4 fluorescent monoclonal antibodies utilize for flow cytometry categorize CD4+ T cells might block CD4 indicate and immobilize relocate T cells in receiver. We have RM4-5 and GK1.5. Appeal notes on BioLegend website speak they together can stop CD4 T cell activation, or even exhaust CD4 T cells.