In addition to understanding the terms in the Glossary you a

In addition to understanding the terms in the Glossary, you are responsible for the material in the Lab Safety, Sterile Technique, Units of Measure and Metric Prefixes, and Scientific Method sections at the beginning of the Lab Manual. Answering the following questions in advance of lab will help you prepare for the quiz. Know the relationships between mole, molecular weight, and molarity. In this lab you will be using 0.1 M HCl. What is the concentration of 0.1 M HCl in mill molar (mM)? How many moles are there in 100 milliliters (mL) of 0.1 M HCl? How many mill moles (mmol)? In Lab 4, you will be using a compound ONPG which has a molecular weight of 300 grams/mol. If you dissolve 30 grams of ONPG in 1 liter of water, what is your final (ONPG) in molar (M)? What is the final concentration in mM? How many grams of ONPG must you dissolve in 1 liter of water to get a solution that is 0.333 M? What is the definition of pH? What is the concentration of hydrogen ions [H^+] in a solution that has a pH of 2? What is the pH of a 0.1 M solution of hydrochloric acid (HC1)? (HC1 is a strong acid, so assume that it dissociates completely in water.) How do changes in pH affect protein structure?

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