Every organism has a characteristic minimum maximum and opti

Every organism has a characteristic minimum, maximum, and optimum growth temperature, as illustrated. To determine the optimum growth temperature for a new isolate, you would perform which of the following? Measure the Generation time at a variety of growth rates, and plot growth Nate versus temperature. Measure the generation time at a variety of temperatures convert the values to rate, and plot growth rate versus growth Perform the streak plate technique, and incubate the plates at a variety of temperatures to see when it grows O Measure the temperature at a variety of generation times, and plot temperature versus generation time.

Solution

The answer is to measure the generation time at a variety of temperatures and then convert it into growth rate and plot growth rate versus temperature

The relation between growth rate and generation time is as follows:-

if N₀=initial population size

N_t= population size at time t

and n=number of generations in time t

then N_t=N₀×2ⁿ

^{taking log to base 10}

^{logN_t=logN₀+n×log2}

^{so n=(logN_t-logN₀)/log2}

^{growth rate constant k= n/t}

so k=(logN_t-logN₀)/t.log2

for generation time t=g and N_t=2N₀

as a result k= [log (2×N₀)-logN₀]/g.log2

k= (log2+logN₀-logN₀)/glog2

k= log2/(g×log2)

k=1/g

hence the growth rates can be calculated from the generation te and then plotted against temperature to measure the optimum growth temperature