How did you arrive at that conclusion lessthanorequalto 3 se

How did you arrive at that conclusion? (lessthanorequalto 3 sentences) You\'ve completed an interrupted mating experiment in E. coli, the leugene enters before the gln gene. For this experiment, you crossed an Hfr that is leu^+ gln^+ with an F- that is leu gln. Exconjugant cultures are plated on minima medium supplemented with leucine but not glutamine, and 250 total colonies were observed. Each those colonies of was then plated on minimal medium containing no leucine or glutamine; only 200 of the cultures grew under these conditions. What are the genotypes of the two different cultures? So, what likely happened at the cellular/molecular level to allow some cell to grow without glutamine supplementation but not all? Make sure you plain/support your answer. (lessthanorequalto 4 sentences)

Solution

1. Genotypes of supplemented medium is leu-, glu+, and in minimal media genotypes are leu+, glu+.

2. At the cellular level: