To answer the following questions read the paper by Pavlukov

To answer the following questions, read the paper by Pavlukov, et al., --> http://www.jbc.org/content/286/26/23296.full.pdf+html

What hypothesis do the authors propose? (2 marks)

Describe apoptosis. (3 marks)

How was survivin initially characterized? What have more recent studies on this protein determined? (4 marks)

Briefly, how did the authors determine the role of the survivin protein in apoptosis? (6 marks)

What were the overall results of their findings? (2 marks)

What is the implication of this study? (2 marks)

Authors consider survivin as major inhibitor of apoptosis and it is most important human genome to cure cancer.

Apoptosis is a process by which cells die due to lack of oxygen and due to infection cells may die.

Firstly survivin was characterized as homodimer and survivin was unable to dimerize.

Survivin monomer was able to interact with smac/DIABLO and smac both invitro and in vivo.

The result of there finding is survivin partly performs its functions as a monomer and partly as a dimer. The mechanism of dimer-monomer balance

regulation may also work as a “switcher” between survivin functions

and thereby explain remarkable functional diversities of this
protein.