What is the minimum number of cycles required to obtain a do

What is the minimum number of cycles required to obtain a double stranded amplification product which starts and ends with the primer sequences you\'ve designed ?

Solution

Double stranded DNA molecules are amplified through polymerase chain reaction (PCR). In this technique, the two strands of DNA are unwound by heating to high temperature of 90⁰C. Then, primers are designed to complementary to 3` end of sequnces and annealed. The primers are extended by DNA polymerase enzyme producing 2 different double stranded DNA molecules. In this, one cycle produced 2 DNA molecules, two cycles produce 4 DNA copies and 3 cycles produce 8 DNA copies and so on. Therefore, the number of cycles required to obtain double stranded amplification product is 1.