URGENT Someone please help need these within the next hour

URGENT - Someone please help, need these within the next hour! Thaanks in advance :)

For this one I am not sure if the true answer is the first or second answer (Xam1 or Xam2). I know the bottom two are false for sure but I\'m not sure about the first two. I say Xam2 since it has the higher Km (hence lower affinity and higher Vmax)...or is it Xam 1 since it has lower Km (hence higher affinity and high vmax)?

Thanks for the help!!!

You take total cell lysate, and use immunoprecipitation to purify an enzyme of interest. The purified enzyme has HIGH specific activity, and shows a single band (56kD) on an SDS PAGE. Now, you heat the total cell lysate, and use immunoprecipitation to purify the enzyme of interest. The purified enzyme has LOW specific activity, and shows two bands (56kD and 122kD) on an SDS PAGE. You then heat the total cell lysate, allow it to cool down, and use immunoprecipitation to purify the enzyme of interest. The purified enzyme has HIGH specific activity, and shows one band (56kD) on an SDS PAGE. Finally, you make total cell lysate from a mutant cell. When you use immunoprecipitation to purify the enzyme of interest, the purified enzyme has LOW specific activity, and shows a single band (56kD) on an SDS PAGE. When you heat the cell lysate from the mutant cells and immunoprecipitate the enzyme, the purified enzyme has Low specific activity, and shows a single band (56kD) on an SDS PAGE. What is the BEST conclusion from this experiment? T The antibody used for immunoprecipitation bound a second protein non-specifically DT F O The 122 kD protein is a chaperone required for proper folding of the 56 kD protein of interest D T The mutant cells make a non-functional protein that does not refold correctly after heating and cooling OT FO? The 122 kD protein prevents the correct folding of the protein under normal conditions. It is a chaperone for refolding after heating.

Solution

Statements of Observation

Normal Cell

1.Cell lysate of purified enzyme has HIGH specific activity, and shows a single band (56kD) on an SDS PAGE.

2.Heat the total cell lysate,enzyme has LOW specific activity, and shows two bands (56kD and 122kD) on an SDS PAGE

3. Heat the total cell lysate, allow it to cool down,purified enzyme has HIGH specific activity, and shows one band (56kD) on an SDS PAGE

Mutant cell.

4. cell lysate from the purified enzyme has LOW specific activity, single band (56kD) on an SDS PAGE

5. After heating the purified enzyme has LOW specific activity, and shows a single band (56kD) on an SDS PAGE.

Conclusion:- The antibody used for immunoprecipitation bound a second protein non-specifically

5. xam2 since it has the higher Km(hence lower affinity and higher Vmax)

Km is the concentration of substrate which allow the enzyme to achieve half Vmax. An enzyme with a high Km value has low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.