Given this partial TEMPLATE DNA sequence below transcribe th

Given this partial TEMPLATE DNA sequence below, transcribe the non-template DNA (remember the RNA would be complimentary to the template and identical (with the exception of U’s for T’s) to the nontemplate strand) and then translate what would be the amino acid sequence. There is no “start” codon given. Fill out the tables with the RNA, the corresponding AA, and then the letter code. Use the letter code to make a final phrase. (Note that there are “stops” for spaces between words and there is no start codon). The amino acid chart is at the end of the document for reference, along with a list of the single letter codes.

3’- AAT AAT CGT TAC CGT AGT ATT CGT GCG CTT ATT ACA GTT TGG CTT ATT -5’

DNA

TTA

TTA

RNA

A.A.

letter

DNA

RNA

A.A.

letter

Final phrase:

3’ – CCT TCT CTT CGT TGG ATC ACC CGC AAA AAG GAT CTC ATT AAG GCT TAA CTT TCA ACT – 5’

DNA

RNA

AA

letter

DNA

CTA

GAG

TAA

TTC

CGA

ATT

GAA

AGT

TGA

RNA

CUA

A.A.

letter

Final phrase:

Need to fill in the blank cells in the table. Could you tell me solution and explain how to get them?

DNA	TTA	TTA
RNA
A.A.
letter

Solution

1.

Template DNA: 3’- AAT AAT CGT TAC CGT AGT ATT CGT GCG CTT ATT ACA GTT TGG CTT ATT -5’

Nontemplate DNA: 5\' - TTA TTA GCA ATG GCA TCA TAA GCA CGC GAA TAA TGT CAA ACC GAA TAA - 3\'

Template DNA: 3’- AAT AAT CGT TAC CGT AGT ATT CGT GCG CTT ATT ACA GTT TGG CTT ATT -5’

RNA:                 5\' - UUA UUA GCA AUG GCA UCA UAA GCA CGC GAA UAA UGU CAA ACC GAA UAA - 3\'

Amino acid sequence: Leu Leu Ala Met Ala Ser STOP Ala Arg Glu STOP Cys Gln Thr Glu STOP

Single letter : L L A M A S A R E C Q T E

Nontemplate DNA: 5\' - TTA TTA GCA ATG GCA TCA TAA GCA CGC GAA TAA TGT CAA ACC GAA TAA - 3\'

RNA:                       3\' - AAU AAU CGU UAC CGU AGU AUU CGU GCG CUU AUU ACA GUU UGG CUU AUU - 5\'

AMINO ACID SEQUENCE: Asn Asn Arg Tyr Arg Ser Ile Arg Ala Leu Ile Thr Val Trp Leu Ile

Single letter : N N R Y R S I R A L I T V W L I

2.

DNA: 3’ – CCT TCT CTT CGT TGG ATC ACC CGC AAA AAG GAT CTC ATT AAG GCT TAA CTT TCA ACT – 5’

DNA: 5\' - GGA AGA GAA GCA ACC TAG TGG GCG TTT TTC CTA GAG TAA TTC CGA ATT GAA AGT TGA - 3\'

DNA: 3’ – CCT TCT CTT CGT TGG ATC ACC CGC AAA AAG GAT CTC ATT AAG GCT TAA CTT TCA ACT – 5’

RNA: 5\' - GGA AGA GAA GCA ACC UAG UGG GCG UUU UUC CUA GAG UAA UUC CGA AUU GAA AGU UGA - 3\'

AMINO ACID SEQUENCE: Gly Arg Glu Ala Thr STOP Trp Ala Phe Phe Leu Glu STOP Phe Arg Ile Glu Ser STOP

Single letter: G R E A T W A F F L E F R I E S

DNA: 5\' - GGA AGA GAA GCA ACC TAG TGG GCG TTT TTC CTA GAG TAA TTC CGA ATT GAA AGT TGA - 3\'

RNA: 3\' - CCU UCU CUU CGU UGG AUC ACC CGC AAA AAG GAU CUC AUU AAG GCU UAA CUU UCA ACU-5\'

AMINO ACID SEQUENCE: Pro Ser Leu Arg Trp Ile Thr Arg Lys Lys Asp Leu Ile Lys Ala STOP Leu Ser Thr

Single letter: P S L R W I T R K K D L I K A l S T