You have generated a T cell clone in which LAT is knocked ou

You have generated a T cell clone in which LAT is knocked out (missing). you stimulate that cell with an epitope loading not the appropriate MHC and test for the IL-2 protein production. Do you expect to detect IL-2 or not? Explain

Solution

Linker for Activation of T cells (LAT) gene plays an important role in the downstream signalling of T-CELL receptor after T-CELL receptor recognises antigen by antigen presenting MHC 1 complex since even if we stimulate it with epitope directly, without MHC CLASS 1. We wont be getting the release of INTERLUKIN 2 because costimulatory signals between antigen presenting cell and T-cell would be missing. AND WITHOUT THOSE CO-STIMULATORY SIGNALS EVEN IF ANTIGEN IS PRESENTED TO T-Cell RECEPTOR DIRECTLY IT WONT GET ACTIVATED AND EVEN IF RECEPTOR GETS ACTIVATED SOME HOW, DOWNSTREAM SIGNALLING WONT OCCUR DUE TO MISSING LAT GENE

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