The concentration of a suspension of Alcaligenes faecalis is
The concentration of a suspension of Alcaligenes faecalis is 8.4 * 105 org/ml.
Answer the following 2 questions using this information.
1. What is the concentration of a suspension with 3 ml of Alcaligenes plus 5 ml of TSB?
2. of 8ml of Alcaligenes plus 10 ml of TSB?
Solution
1. What is the concentration of a suspension with 3 ml of Alcaligenes plus 5 ml of TSB?
Answer:
Stock: 8.4 * 10^5 org/ml.
Fold Dilution= (3mL + 5mL)/3mL
= 2.6
Final concentration = stock/ fold dilution
=(8.4 * 10^5)/2.6
=3.2 x 10^5 org/ml.
2. of 8 ml of Alcaligenes plus 10 ml of TSB?
Answer:
Fold Dilution= (8mL + 10mL)/8mL
= 2.25
Final concentration = stock/ fold dilution
=(8.4 * 10^5)/2.25
=3.73 x 10^5 org/ml.
 =

