You perform a colorimetric enzyme assay to determine the act

You perform a colorimetric enzyme assay to determine the activity of inverts in a bioreactor (total volume = 1L) used to produce inverted sugar. You prepare your standard curve by mixing known monosaccharide dilutions (3ml) with the DNS reagent (2ml). The standard curve follows the following linear function: Absorbance (540nm) = 1.82 X monosaccharide concentration (M) You mix 1.5ml of the enzyme solution from the bioreactor with 1.5ml of substrate solution and let them react for exactly 10 min, after which you stop the reaction by adding 2ml of DNS reagent. You then add 20ml of distilled water and read the absorbance at 540nm. You obtain an absorbance value of 0.395. Determine the reaction rate (mol monosaccharide formed/min) in the bioreactor.

Solution

Answer:

Rate of any reaction is determined by following equation-----

Rate=k[A]a[B]b

Where,[A] and [B] are concentration of the solution and a and b are order of reaction. The mixture of any solution can be calculated through this formula. The rate can be determined at different time points. However, k or rate constant value should be known. Here in the present dataset k value is absent. The remaining concentration could be calculated by this formula-- concentration of A= quantity of A /given medium.

Mireover, the reaction rate in this present problem could be decided into two half\'s.. Initial rate and reaction rate after 10 min.