Explain why an antibody raised against a transition state an

Explain why an antibody raised against a transition state analogue may catalyze a reaction involving that transition state. Using the transition state analog of an ester. Why would this approach not be suitable for finding an antibody which would hydrolyse the phosphodiester bond?

Solution

An antibody raised against a transition state analogue may catalyze a reaction involving that the transition state analog of an ester because these are these are catalytic antibodies & they are enzymatic ally active. So that they are going to convert the transition state analog of an ester into products by binding to stable binding site further stabilizes transition state so that referred as \"abzymes\" & they are pentavalent thus reduce the activation energy of transition state analog.

Catalytic efficiency of the antibodies towards the transition state analog of an ester is highly lower so that the this approach not be suitable for finding an antibody which would hydrolyse the phosphodiester bond & it is very limited due to physical constraints 7 hydrolysis reactions.