Outline an experiment that can be done to test this correlat

Outline an experiment that can be done to test this correlation between methylation and tumor evolution. Be specific. What specific areas are important for future clarifications?

Solution

DNA methylation off the gene expression which also affects translation of tumor suppressor genes and then cells can develop into tumor. DNA demethylation may helpful to reduce the life span of tumor cells. Hypermethylation condition reflects the tumor causing or tumor conditions. Hypermethylated promoters serve as biomarkers for such cells. Hypermethylation silences tumor suppressor genes. DNA methylation may play a role in regulating expression of noncoding RNAs which will helpful in tumor suppression. DNA methylation is a reversible process which makes it more convenient tool for diagnosis and treatment of cancer. Therefore, researchers use demethylation process to reduce the mehtylated DNA. That is helpful for some time. A future threat is that the process may increase the speed of tumor progression. The effect of chemotherapeutic agents and survival rate calculate by DNA methylation profile. For future, the method needs to develop in noninvasive or minimally invasive procedures; its markers have greater sensitivity and specificity. Researchers are progress to make more potent the demethylating drugs, some drugs as azacytidine, fazarabine and decitabine are already in use.