is the PDB entry code 5L9W Apc core complex a full length pr

is the PDB entry code 5L9W (Apc core complex) a full length protein? Or it is domain or some trucncated form of the larger protein , and if so what is the domain layout of the full-length protein? What does the studied construct natively bind to?

Solution

PDB or Protein Data Bank is a crystallographic database for proteins and nucleic. It stores three-dimensional structural data of these large biological molecules. The data is collected by NMR spectroscopy, cryo-electron microscopy and X-ray crystallography etc.

5L9W is not a full length protein but major portion of the protein and forms core complex of the protein (acetophenone carboxylase). The Acetophenone carboxylase found in various microorganisms to detoxify toxic compounds.

Acetophenone carboxylase is an enzyme that catalyses ATP- dependent carboxylation of Acetophenone:

2 ATP + acetophenone + HCO₃ + H₂O + H⁺ {\\displaystyle \ ightleftharpoons }   à2 ADP + 2 phosphate + 3-oxo-3-   phenylpropanoate

Acetophenone carboxylase is a large protein complex which has two components heterooctameric core complex (’)₂(apc core complex, 5L9W) of 482 kDa and apc 34kDa. It also contain two Zn²⁺ ions. In the apc core complex apc and apc’ forms the ATP binding modules and acts as ATP–dependent kinases.