Homework SourseThe table below presents the results of different digests of
The table below presents the results of different digests of a plasmid. All sizes are in base pairs.
1. One of the enzymes used only partially digested the DNA. Which enzyme is it?
2. Indicate the size in base pairs of one of the intermediate products which represents an incompletely digested DNA fragment in the double digests.
3. What is the total plasmid size in base pairs?
4. What are the distances between the KpnI and NheI restriction sites?
5. What are the distances between the BamHI and NheI restriction sites?
6. What are the distances between the BamHI and KpnI restriction sites
| BamHI | 13199,8572,4627 |
| KpnI | 13199 |
| NheI | 13199 |
| BamHI + KpnI | 12126, 9645, 8572, 3554, 1073 |
| BamHI + NheI | 10701, 9645, 8572, 2498, 2129 |
| NheI + KpnI | 11774, 1425 |
Solution
The distances between the KpnI + NheI restriction sites- 1425.
The distances between the BamHI and NheI restriction sites- 10701.
The distances between the BamHI and KpnI restriction sites -1425.
