Through SDSPAGE it is possible to a determine a proteins iso

Through SDS-PAGE, it is possible to:

a. determine a protein\'s isoelectic point

b. determine an enzyme\'s specific activity

c. determine the amino acid composition of the protein

d. preserve a protein\'s native structure and biological activity

e. seperate proteins exclusively on the basis of their size

Solution

Answer : The correct answer is E (seperate proteins exclusively on the basis of their size)

Sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) is a molecular biology technique which is routinely used for the separation and determination of molecular weights of proteins. Movement of different proteins is guided by the size of the proteins. In SDS-PAGE smaller proteins move faster compared to large proteins and forms separate bands on the gels.