Bacteria that is transformed with a circular pYESCTLacZ vect

Bacteria that is transformed with a circular pYES/CT/LacZ vector (without GOI) will:

A)Not survive on an Ampicillin plate because it does not contain the GOI

B)Survive on an Ampicillin plate and be white

C)Survive on an Ampicillin plate and be blue

D)Not survive on an Ampicillin plate because the promotor for the Ampicillin resistance gene will not have been activated.

E)Not survive on an Apicillin plate because the CYC1 sequence will not have been activated

F)Not survive on an Ampicillin plate because it contains the LacZ gene

Solution

pYES/CT/LacZ vector contains the ampicillin gene. Now, since gene of interest is absent, so ampicillin resistance gene is not distorted. So, this bacteria will survive on ampicillin plate.

Second, LacZ gene is there, which will produce beta-galactosidase. In presence of X-gal, cells transformed with non-recombinant plasmids (i.e. only the vector) always grow into blue colonies.

Therefore, \'C\' is the correct answer.