1 Your laboratorys protocol for starting mammalian cultures

1. Your laboratory’s protocol for starting mammalian cultures is to have an initial density of cells of 500,000 cells/mL. If you are starting a 250mL culture and given a set of cryovials that contain approximately 1 million cells each; how many cryovials will you need to reach the minimum target starting density?

2. You have been tasked with setting up a cell bank of frozen cell cryovials for a cell line you’ve been working with. The goal is to get at least 1000 cryovials that each contain 2.5-million cells per vial. You only have 3-L bioreactors to work with to culture all the cells needed. Assuming that you will harvest the bioreactors when the cells reach 1-million cells per mL, how many bioreactor cultures will you need to get the desired number of cryovials?

Solution

(1)

Answer:

We need 500,000 cells/mL

Volume required: 250 mL

Total cells required = 250 x 500,000 = 125,000,000 cells (125 million cells)

Stock of cells 1million cells/cryovial

We need a total of 125 cryovials to reach the minimum target starting density