Please complete the following question 7 Troubleshooting gel

Please complete the following question:

7. Troubleshooting gels\" What effect would be observed in the strained bands of DNA in an agarose gel....

A. if the casting tray is moved or jarred while the agarose is solidifying
B. if the gel is run at very high voltage?
C. if a large air bubble or clump of debris is allowed to set in the agarose?
D. if too much DNA is loaded in a lane?
E. if there is incomplete (partial) digestion by a restriction enzyme?

Solution

A. In this case, gel is not homogeneous in agarose content, so the bands that appear \"dissorted\". It will not be in horizontal position.

B.The might gets melt if it runs in high voltage.

C.If the DNA run into the bubble and it\'s hard to estimate the exact sie of the DNA fragment. In case, the size of the bubble is bigger it might even diffuse into the buffer.

D. We can see the big band and it runs faster than their true size.

E.We can see the sheared band and we cannot estimate the size exactly.