Describe a way to incorporate tyrosine instead of the normal

“Describe a way to incorporate “tyrosine” instead of the normal stop codon “UAG” in E. coli.”

Solution

E.coli is a gram negative bacteria contains DNA. E.coli is used in production of many proteins in large scale. usually inmany research purposes E.Coli uses for production for therapeutic proteins for treating several diseases. E.coli is favorable bacteria to cause gentic mutations for several purposes. usually in protein synthesis RNA plays important role. in the process of protein synthesis polypeptide formation occurs by transcription, translatio. by adding UAA, UAG, UGA (non sense condon) act as terminating or stop codons which terminate the message for the synthesis of a polypeptide which is required for protein synthesis. so here replacing the UAG with TYROSINE causing genetic mutations which are called as non sense mutations. these mutations may lead to uncontrolled peptide generation. these kind of mutation created for the purpose of therapeutic proteins production. example : insulin for diabetes, human growth hormones for growth deficiencies.