1 The DNA sequence below was used as a template for producin

1. The DNA sequence below was used as a template for producing RNA, GGTATAT CCG TTC a. Below the DNA, type the mRNA sequence transcribed and appropriately label the ends as 5\' or b. If the DNA sequence above was used as a template for producing RNA, would it be the coding strand or anticoding strand? c. Write down the tRNA sequences for each codon. 2. How many amino acids are encoded by the following portion of mRNA sequence (do not count start and stop codons, if any) CAUACCUCACCACUUGGUUGA. 3. Consider the following sequence of genomic DNA which is a template strand containing the beginning of the open reading frame for a gene TGTACAAAAAGGGcCA TAAA ACT (a) Type the coding strand above the template strand and label the 5\' and 3\' ends. (b) Find the open reading frame on the template strand and identify the start codon by circling it. (b) Below the DNA, transcribe the open reading frame in a strand of mRNA. Circle the start to codon (c) What is the amino acid sequence of the peptide encoded by this strand of mRNA?

Solution

Q. I

Answer

The DNA sequence is 3\' GGTATATCCGGGAAATTC 5\'

The Coding mRNA will be   5\' CCAUAUAGGCCCUUUAAG 3\'

The RNA transcript is synthesized on the template strand (Non-Coding) and is identical in sequence (with U in place of T) to the nontemplate strand, or coding strand. Therefore, if the DNA sequence above was used as a template for producing RNA, it would be Anti (Non)-Coding Strand

The tRNA sequences for the transcript   5\' CCA UAU AGG CCC UUU AAG 3\'

at the Anti-codon region would be 3\' GGU AUA UCC GGG AAA UUUC 5\'

Therefore, 6 different types of tRNAs will be used to translate this codes from mRNA molecule