two methods that a cell may employ y to be certain that it h

two methods that a cell may employ y to be certain that it has uracil

Solution

Uracil is a nitrogenous base found in RNA. It is present in place of thymine. It is not found in DNA. If it is found in DNA, it means that it is a mismatch, which should be repaired.

To find out the presence of Uracil in DNA of a cell, one method is:

1. Treatment with DNA glycosylase/ Uracil DNA glycosylase: This enzyme removes uracil. This enzyme cleaves uracil from the region of DNA where it is present; but does not remove this from RNA. The method is more specifically known as Base-excision repair. So, you uracil glycosylase assay or ELISA using this enzyme can track uracil in DNA.

2. Cytosine deamination: This process produces uracil from cytosine. Again treatment with uracil glycosylase will remove the uracil. So, through deamination of cytosine, which is a natural process in the cell, the cell will know that it has wrongly employed uracil in its DNA.

*** Hope it helps.