CONDUCTING HEMOGLOBIN ELECTROPHORESIS TESTING DIAGNOSING SIC

CONDUCTING HEMOGLOBIN ELECTROPHORESIS TESTING: DIAGNOSING SICKLE CELL ANEMIA

Patient 1 has HbA and HbF, patient 2 has HbA and HbS. The question is describe the phenotype of the blood cells of your two patients you analyzed by hemoglobin electrophoresis assay. List all hemoglobin protein type(s) you would expect to be present in patient 1 and in patient 2. Indicate if disease symptoms would be absent or present (indicate severity).

Solution

Answer:

a. Phenotype of RBCs:

HbAF: Normal

HbAS: Some normal hemoglobin and some \"sickle\" hemoglobin is produced

b. The hemoglobin proteins seen in the samples:

HbAF: Hemoglobin A, Hemoglobin F, Hemoglobin A2, Carbonic anhydrase

HbAS: Hemoglobin A, Hemoglobin S, Hemoglobin A2, Carbonic anhydrase

c. Disease symptoms:

HbAF: No disease symptoms

HbAS: Heterozygotes of HbA with HbS have the Sickle Cell Trait (carrier of HbS). So, the patient will show mild form of the disease.