Design an experiment to determine the LD50 and the NOAEL for

Design an experiment to determine the LD50 and the NOAEL for Egyptian cobra venom.

Solution

The LD50 is defined as the dose of a drug/toxin at which it kills the 50% of cells in culture. The NOAEL is defined as the no-observed-adverse-effect-level of the drug and deciphers the highest concentration of the drug at which it shows no adverse/lethal/cytotoxic effects. In order to determine these parameters, following experiment can be planned:

Thus, by performing the above mentioned assay, the LD50 and NOAEL of the snake venom can be estimated.