1 How much trypsin do you use to treat a 6well plate 2 At wh

1. How much trypsin do you use to treat a 6-well plate?

2. At what density of cells should one start a new plate when doing a cell culture?

3. What happens to fibroblasts if they are cultured “beyond confluence”?

Solution

1- 2 ml of 0.05% trypsin is added in 6 well plate.

2-Density of cells after which new plate is to be plated is 3 X 10⁵.

3- Beyond confluence, growth rate of cells get reduced.