In 250300 words describe three properties common to the reac

In 250-300 words, describe three properties common to the reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase, and RNA replicase. How is the enzyme polynucleotide phosphorylase similar to and different from these four enzymes?

Solution

1. The common properties of reactions catalyzed by DNA polymerase, RNA polymerase, reverse transcriptase and RNA replicase are: a) The synthesis occurs in a 5\' - 3\' direction and also there is formation of phosphodiester bond in between nucleotides. b) The sequence which is synthesized is complementary to the template, hence the reaction is template directed. and c) The reactions releases pyrophosphate as a product.

2. The properties of polynucleotide phosphorylase which differs from other four enzymes are: a) The reaction catalyzed by polynucleotide phosphorylase is not template directed. b) Also the synthesis occurs in 3\' - 5\' direction.

3. The property of polynucleotide phosphorylase which is similar to other four enzymes is: a) The formation of phosphodiester bond in between nucleotide.